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芯片及抗体专区

细胞凋亡抗体芯片

 RayBio® Apoptosis Array

细胞凋亡和疾病的关系

         I型糖尿病

         神经退行性疾病:老年痴呆、帕金森氏症、亨廷顿氏症等

         心肌梗塞

         艾滋病

         癌症

         自身免疫

         长期感染

Product Highlights

           Relative level of 43 apoptosis –related markers are detected simultaneously

           No need to perform individual immunoprecipitations or Western Blot

           Reliable results with wide range of cell lines

           High specificity, sensitivity and reproducibility

           No special equipment required

           Many choices: chemiluminescence or fluorescence detection system; Membrane or glass chip

Contents of kit

          RayBio® Human Apoptosis Antibody Array kit membranes (2, 4, or 8 membranes)

          2X Cell Lysis Buffer (5 ml)

          Protease Inhibitor Cocktail (1 tube for 2-4 membranes, and 2 for 8 membranes)

          Blocking Buffer (25 ml for less 4 membranes and 50 ml for 8 membranes)

          20X Wash Buffer I (30 ml)

          20X Wash Buffer II (30 ml)

          Cocktail of Biotin-Conjugated Antibody Mix (1 tube for 2 membranes, 2 for 4 membranes, and 4 for 8 membranes)

          1,000X HRP-Conjugated Streptavidin (18 µl)

          Detection Buffer C (1.5 ml for 2~4 membranes, 2.5 ml for membranes)

          Detection Buffer D (1.5 ml for 2~4 membranes, 2.5 ml for 8 membranes)

          Eight-Well Tray (1 each)

 

原理:

 

Representative Results

 

Apoptotic protein profiling in drug-treated Jurkat cultured cells: Jurkat cells were treated with Apoptosis Inducer Set (BioVision, Inc. cat# K121-5) for 7 hours (10mM Actinomycin D, 2mM Camptothecin, 100mM Cycloheximide, 10mM Dexamethasone, and 100mM Etopiside). 500 mg of cell lysates from both induced and uninduced Jurkat cells were incubated overnight with RayBio® Human Apoptosis Antibody Array membranes. Control membrane was incubated with blocking buffer. The membranes were then washed and cocktail of biotinylated antibodies was used to detect bound proteins. After incubation with HRP-streptavidin, the signals were visualized by chemiluminescence.

Apoptotic protein profiling in drug-treated Jurkat cultured cells Array data is confirmed by Western Analysis

Map
  1 2 3 4 5 6 7 8 9 10 11 12 13 14
1 Pos Pos Neg Neg Blank Blank bad bax bcl-2 bcl-w BID BIM Caspase3 caspase8
2 Pos Pos Neg Neg Blank Blank bad bax bcl-2 bcl-w BID BIM Caspase3 caspase8
3 CD40 CD40L cIAP-2 cytoC DR6 Fas FasL Blank HSP27 HSP60 HSP70 HTRA IGF-I IGF-II
4 CD40 CD40L cIAP-2 cytoC DR6 Fas FasL Blank HSP27 HSP60 HSP70 HTRA IGF-I IGF-II
5 IGFBP-1 IGFBP-2 IGFBP-3 IGFBP-4 IGFBP-5 IGFBP-6 IGF-1sR blank livin p21 p27 p53 SMAC Survivin
6 IGFBP-1 IGFBP-2 IGFBP-3 IGFBP-4 IGFBP-5 IGFBP-6 IGF-1sR blank livin p21 p27 p53 SMAC Survivin
7 sTNF-R1 sTNF-R2 TNF-alpha TNF-beta TRAILR-1 TRAILR-2 TRAILR-3 TRAILR-4 XIAP Blank Neg Neg Neg Pos
8 sTNF-R1 sTNF-R2 TNF-alpha TNF-beta TRAILR-1 TRAILR-2 TRAILR-3 TRAILR-4 XIAP Blank Neg Neg Neg Pos

价格:

Membrane-Based Arrays: 

Product Cat# Size Detection Species Sample type Price
RayBio® Human Apoptosis Antibody Array  (2) AAH-APO-1-2 2 membranes for 2 samples Chemiluminescence Human Cell lysates 2980
RayBio® Human Apoptosis Antibody Array  (4) AAH-APO-1-4 4 membranes for 4 samples Chemiluminescence Human Cell lysates 4890
RayBio® Human Apoptosis Antibody Array  (8) AAH-APO-1-8 8 membranes for 8 samples Chemiluminescence Human Cell lysates 8800
RayBio® Human Apoptosis Antibody Array  Service AAH-APO-SV Minumum 2 samples Chemiluminescence Human Cell lysates Inquiry

Glass Chip-Based Arrays: 

Product Cat# Size Detection Species Sample type Price
RayBio® Human Apoptosis Antibody Array  (4) AAH-APO-G1-4 1slide for 4 samples Fluorescence Human Cell lysates 4280
RayBio® Human Apoptosis Antibody Array  (8) AAH-APO-G1-8 2 slides for 8 samples Fluorescence Human Cell lysates 7280
RayBio® Human Apoptosis Antibody Array  Service AAH-APO-GSV Minumum 4 samples Fluorescence Human Cell lysates Inquiry

更多产品信息请联系:

广州聚研生物科技有限公司

  话:86-20-87322722/ 87328692

  真:86-20-87328692

  址:http://www.asbio.com.cn/

  址:广州市中山一路51号拓业大厦506室(510600
E-mailinfo@asbio.com.cn

 

发布日期:2010-9-1 【返回】